Measurement and Study of Partitioning of Arginine and Glycine in Aqueous Two-Phase Systems Containing Polyethylene Glycol and Salt

Document Type : Research paper

Authors

1 Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran

2 Department of Chemical Engineering, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran

3 Chemical Engineering, Amirkabir University of Technology, Iran, Tehran

Abstract

In this study, the partitioning of glycine and arginine in aqueous two-phase systems (ATPSs) was measured. The aqueous two-phase systems containing polyethylene glycol with molecular weights of 8000 and 6000 (Da) and trisodium citrate and sodium sulfate were investigated. These systems have been studied at three different temperatures of 298, 303, and 308 K. The effect of different weight percentages of salt, the weight percentages of polymer, and temperature on the partitioning of glycine and arginine at ambient pressure was investigated. The results showed that the partition coefficients of glycine and arginine decreased with an increasing weight percentage of salt and polymer. Also, as the tie line length increases, the partitioning of amino acids decreases. The results showed that glycine in ATPS containing sodium sulfate (10 wt%) and polyethylene glycol (15 wt%, molecular weight 8000 Da) at 308 K has the highest partition coefficient in the top phase (2.013 ±0.101). Also, arginine in ATPS containing sodium triscitrate (14 wt%) and polyethylene glycol (25 wt%, molecular weight 6000 Da) at 308 K has the best partition coefficient (0.059 ± 0.005) in the bottom phase.

Keywords

Journal of Separation Science and Engineering
Volume 13, Issue 1
September 2021
Pages 57-70

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